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Pierce,AminoLink Reductant

货号:44892,名称: AminoLink Reductant ,品牌:PIERCE,规格:2 X 1 G
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AminoLink Reductant, 2 x 1 g 介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

 

AminoLink Reductant, 2 x 1 g 现货供应

 

PIERCE

44892

AminoLink Reductant, 2 x 1 g.

2 X 1 G

PIERCE

44894

AminoLink Plus Immobilization Kit

1 KIT

PIERCE

44897

IgM Purification Kit

EA

 

AminoLink Reductant, 2 x 1 g 品牌介绍

美国Pierce公司(www.pierce.com)是蛋白质化学、免疫学和层析等很多方面的先驱和领导者。http://www.thermo.com.cn/Category828.html

 

主要产品包括:

1)化学发光体和比色底物

 

2)分析试剂

 

3)荧光标记探针

 

4)酶联免疫分析试剂盒等

 

AminoLink Reductant, 2 x 1 g 仅用于科研

 

Thermo Scientific AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

Proteins and other molecules with primary amines can be covalently attached (immobilized) to AminoLink Resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.

 

Highlights:

  • AminoLink Coupling Resin – aldehyde-activated crosslinked, 4% beaded agarose
  • Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH2)
  • Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
  • Stable, permanent immobilization – Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
  • Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
  • Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions



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